Tomato line fir-A818-0433

ABSTRACT

The invention provides seeds and plants of tomato line FIR-A818-0433. The invention thus relates to the plants, seeds, plant parts, and tissue cultures of tomato line FIR-A818-0433 and to methods for producing a tomato plant produced by crossing such plants with themselves or with another plant, such as a tomato plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to plants, seeds, plant parts, and tissue cultures of tomato line FIR-A818-0433 comprising introduced beneficial or desirable traits.

FIELD OF THE INVENTION

The present invention relates to the field of plant breeding and, morespecifically, to the development of tomato hybrid SVTH3452, tomato lineFIR-A818-0433, and tomato line FDR-A815-3562V.

BACKGROUND OF THE INVENTION

The goal of vegetable breeding is to combine various desirable traits ina single variety. Such desirable traits may include any trait deemedbeneficial or desirable by a grower or consumer, including greateryield, resistance to insects or disease, tolerance to environmentalstress, and nutritional value.

Breeding techniques take advantage of a plant's method of pollination.There are two general methods of pollination: a plant self-pollinates ifpollen from one flower is transferred to the same or another flower ofthe same plant or plant variety. A plant cross-pollinates if pollencomes to it from a flower of a different plant variety.

Plants that have been self-pollinated and selected for type over manygenerations become homozygous at almost all genetic loci and produce auniform population of true breeding progeny, a homozygous plant. A crossbetween two such homozygous plants of different genotypes produces auniform population of hybrid plants that are heterozygous for manygenetic loci. Conversely, a cross of two plants each heterozygous at anumber of loci produces a population of hybrid plants that differgenetically and are not uniform. The resulting non-uniformity makesperformance unpredictable.

The development of uniform varieties requires the development ofhomozygous inbred plants, the crossing of these inbred plants, and theevaluation of the crosses. Pedigree breeding and recurrent selection areexamples of breeding methods that have been used to develop inbredplants from breeding populations. Those breeding methods combine thegenetic backgrounds from two or more plants or various other broad-basedsources into breeding pools from which new lines and hybrids derivedtherefrom are developed by selfing and selection of desired phenotypes.The new lines and hybrids are evaluated to determine which of those havecommercial potential.

SUMMARY OF THE INVENTION

In one aspect, the present invention provides a tomato plant of hybridSVTH3452, line FIR-A818-0433 or line FDR-A815-3562V. Also provided aretomato plants having all the physiological and morphologicalcharacteristics of such a plant. Parts of these tomato plants are alsoprovided, for example, including pollen, an ovule, an embryo, a seed, ascion, a rootstock, a fruit, and a cell of the plant.

In another aspect of the invention, a plant of tomato hybrid SVTH3452,tomato line FIR-A818-0433, or tomato line FDR-A815-3562V comprising anadded heritable trait is provided. The heritable trait may comprise agenetic locus that is, for example, a dominant or recessive allele. Inone embodiment of the invention, a plant of tomato hybrid SVTH3452,tomato line FIR-A818-0433, or tomato line FDR-A815-3562V is defined ascomprising a single locus conversion. In specific embodiments of theinvention, an added genetic locus confers one or more traits such as,for example, herbicide tolerance, insect resistance, disease resistance,and modified carbohydrate metabolism. In further embodiments, the traitmay be conferred by a naturally occurring gene introduced into thegenome of a line by backcrossing, a natural or induced mutation, or atransgene introduced through genetic transformation techniques into theplant or a progenitor of any previous generation thereof. Whenintroduced through transformation, a genetic locus may comprise one ormore genes integrated at a single chromosomal location.

In some embodiments, a single locus conversion includes one or moresite-specific changes to the plant genome, such as, without limitation,one or more nucleotide modifications, deletions, or insertions. A singlelocus may comprise one or more genes or nucleotides integrated ormutated at a single chromosomal location. In one embodiment, a singlelocus conversion may be introduced by a genetic engineering technique,methods of which include, for example, genome editing with engineerednucleases (GEEN). Engineered nucleases include, but are not limited to,Cas endonucleases; zinc finger nucleases (ZFNs); transcriptionactivator-like effector nucleases (TALENs); engineered meganucleases,also known as homing endonucleases; and other endonucleases for DNA orRNA-guided genome editing that are well-known to the skilled artisan.

The invention also concerns the seed of tomato hybrid SVTH3452, tomatoline FIR-A818-0433, or tomato line FDR-A815-3562V. The seed of theinvention may be provided as an essentially homogeneous population ofseed of tomato hybrid SVTH3452, tomato line FIR-A818-0433, or tomatoline FDR-A815-3562V. Essentially homogeneous populations of seed aregenerally free from substantial numbers of other seed. Therefore, seedof tomato hybrid SVTH3452, tomato line FIR-A818-0433, or tomato lineFDR-A815-3562V may be defined as forming at least about 97% of the totalseed, including at least about 98%, 99%, or more of the seed. The seedpopulation may be separately grown to provide an essentially homogeneouspopulation of tomato plants designated SVTH3452, FIR-A818-0433, orFDR-A815-3562V.

In yet another aspect of the invention, a tissue culture of regenerablecells of a tomato plant of hybrid SVTH3452, tomato line FIR-A818-0433,or tomato line FDR-A815-3562V is provided. The tissue culture willpreferably be capable of regenerating tomato plants capable ofexpressing all of the physiological and morphological characteristics ofthe starting plant and of regenerating plants having substantially thesame genotype as the starting plant. Examples of some of thephysiological and morphological characteristics of tomato hybridSVTH3452, tomato line FIR-A818-0433, or tomato line FDR-A815-3562Vinclude those traits set forth in the tables herein. The regenerablecells in such tissue cultures may be derived, for example, from embryos,meristems, cotyledons, pollen, leaves, anthers, roots, root tips,pistils, flowers, seed, and stalks. Still further, the present inventionprovides tomato plants regenerated from a tissue culture of theinvention, the plants having all the physiological and morphologicalcharacteristics of tomato hybrid SVTH3452, tomato line FIR-A818-0433, ortomato line FDR-A815-3562V.

In still yet another aspect of the invention, processes are provided forproducing tomato seeds, plants, and fruit, which processes generallycomprise crossing a first parent tomato plant with a second parenttomato plant, wherein at least one of the first or second parent plantsis a plant of tomato line FIR-A818-0433 or tomato line FDR-A815-3562V.These processes may be further exemplified as processes for preparinghybrid tomato seed or plants, wherein a first tomato plant is crossedwith a second tomato plant of a different, distinct genotype to providea hybrid that has, as one of its parents, a plant of tomato lineFIR-A818-0433 or tomato line FDR-A815-3562V. In these processes,crossing will result in the production of seed. The seed productionoccurs regardless of whether the seed is collected or not.

In one embodiment of the invention, the first step in “crossing”comprises planting seeds of a first and second parent tomato plant,often in proximity so that pollination will occur for example, mediatedby insect vectors. Alternatively, pollen can be transferred manually.Where the plant is self-pollinated, pollination may occur without theneed for direct human intervention other than plant cultivation.

A second step may comprise cultivating or growing the seeds of first andsecond parent tomato plants into plants that bear flowers. A third stepmay comprise preventing self-pollination of the plants, such as byemasculating the flowers (i.e., killing or removing the pollen).

A fourth step for a hybrid cross may comprise cross-pollination betweenthe first and second parent tomato plants. Yet another step comprisesharvesting the seeds from at least one of the parent tomato plants. Theharvested seed can be grown to produce a tomato plant or hybrid tomatoplant.

The present invention also provides the tomato seeds and plants producedby a process that comprises crossing a first parent tomato plant with asecond parent tomato plant, wherein at least one of the first or secondparent tomato plants is a plant of tomato hybrid SVTH3452, tomato lineFIR-A818-0433, or tomato line FDR-A815-3562V. In one embodiment of theinvention, tomato seed and plants produced by the process are firstgeneration (F₁) hybrid tomato seed and plants produced by crossing aplant in accordance with the invention with another, distinct plant. Thepresent invention further contemplates plant parts of such an F₁ hybridtomato plant, and methods of use thereof. Therefore, certain exemplaryembodiments of the invention provide an F₁ hybrid tomato plant and seedthereof.

In still yet another aspect, the present invention provides a method ofproducing a plant derived from tomato hybrid SVTH3452, tomato lineFIR-A818-0433, or tomato line FDR-A815-3562V, the method comprising thesteps of: (a) preparing a progeny plant derived from tomato hybridSVTH3452, tomato line FIR-A818-0433, or tomato line FDR-A815-3562V,wherein said preparing comprises crossing a plant of tomato hybridSVTH3452, tomato line FIR-A818-0433, or tomato line FDR-A815-3562V witha second plant; and (b) crossing the progeny plant with itself or asecond plant to produce a seed of a progeny plant of a subsequentgeneration. In further embodiments, the method may additionallycomprise: (c) growing a progeny plant of a subsequent generation fromsaid seed of a progeny plant of a subsequent generation and crossing theprogeny plant of a subsequent generation with itself or a second plant;and repeating the steps for an additional 3-10 generations to produce aplant derived from tomato hybrid SVTH3452, tomato line FIR-A818-0433, ortomato line FDR-A815-3562V. The plant derived from tomato hybridSVTH3452, tomato line FIR-A818-0433, or tomato line FDR-A815-3562V maybe an inbred line, and the aforementioned repeated crossing steps may bedefined as comprising sufficient inbreeding to produce the inbred line.In the method, it may be desirable to select particular plants resultingfrom step (c) for continued crossing according to steps (b) and (c). Byselecting plants having one or more desirable traits, a plant derivedfrom tomato hybrid SVTH3452, tomato line FIR-A818-0433, or tomato lineFDR-A815-3562V is obtained which possesses some of the desirable traitsof the line/hybrid as well as potentially other selected traits.

In certain embodiments, the present invention provides a method ofproducing food or feed comprising: (a) obtaining a plant of tomatohybrid SVTH3452, tomato line FIR-A818-0433, or tomato lineFDR-A815-3562V, wherein the plant has been cultivated to maturity, and(b) collecting at least one tomato from the plant.

In still yet another aspect of the invention, the genetic complement oftomato hybrid SVTH3452, tomato line FIR-A818-0433, or tomato lineFDR-A815-3562V is provided. The phrase “genetic complement” is used torefer to the aggregate of nucleotide sequences, the expression of whichsequences defines the phenotype of, in the present case, a tomato plant,or a cell or tissue of that plant. A genetic complement thus representsthe genetic makeup of a cell, tissue or plant, and a hybrid geneticcomplement represents the genetic make-up of a hybrid cell, tissue orplant. The invention thus provides tomato plant cells that have agenetic complement in accordance with the tomato plant cells disclosedherein, and seeds and plants containing such cells.

Plant genetic complements may be assessed by genetic marker profiles,and by the expression of phenotypic traits that are characteristic ofthe expression of the genetic complement, e.g., isozyme typing profiles.It is understood that tomato hybrid SVTH3452, tomato line FIR-A818-0433,or tomato line FDR-A815-3562V could be identified by any of the manywell-known techniques such as, for example, Simple Sequence LengthPolymorphisms (SSLPs) (Williams et al., Nucleic Acids Res., 18:6531-6535, 1990), Randomly Amplified Polymorphic DNAs (RAPDs), DNAAmplification Fingerprinting (DAF), Sequence Characterized AmplifiedRegions (SCARs), Arbitrary Primed Polymerase Chain Reaction (AP-PCR),Amplified Fragment Length Polymorphisms (AFLPs) (EP 534 858,specifically incorporated herein by reference in its entirety), andSingle Nucleotide Polymorphisms (SNPs) (Wang et al., Science,280:1077-1082, 1998).

In still yet another aspect, the present invention provides hybridgenetic complements, as represented by tomato plant cells, tissues,plants, and seeds, formed by the combination of a haploid geneticcomplement of a tomato plant of the invention with a haploid geneticcomplement of a second tomato plant, preferably, another, distincttomato plant. In another aspect, the present invention provides a tomatoplant regenerated from a tissue culture that comprises a hybrid geneticcomplement of this invention.

Any embodiment discussed herein with respect to one aspect of theinvention applies to other aspects of the invention as well, unlessspecifically noted.

The term “about” is used to indicate that a value includes the standarddeviation of the mean for the device or method being employed todetermine the value. The use of the term “or” in the claims is used tomean “and/or” unless explicitly indicated to refer to alternatives onlyor the alternatives are mutually exclusive. When used in conjunctionwith the word “comprising” or other open language in the claims, thewords “a” and “an” denote “one or more,” unless specifically notedotherwise. The terms “comprise,” “have,” and “include” are open-endedlinking verbs. Any forms or tenses of one or more of these verbs, suchas “comprises,” “comprising,” “has,” “having,” “includes,” and“including,” are also open-ended. For example, any method that“comprises,” “has,” or “includes” one or more steps is not limited topossessing only those one or more steps and also covers other unlistedsteps. Similarly, any plant that “comprises,” “has,” or “includes” oneor more traits is not limited to possessing only those one or moretraits and covers other unlisted traits.

Other objects, features, and advantages of the present invention willbecome apparent from the following detailed description. It should beunderstood, however, that the detailed description and any specificexamples provided, while indicating specific embodiments of theinvention, are given by way of illustration only, since various changesand modifications within the spirit and scope of the invention willbecome apparent to those skilled in the art from this detaileddescription.

DETAILED DESCRIPTION OF THE INVENTION

The invention provides methods and compositions relating to plants,seeds, and derivatives of tomato hybrid SVTH3452, tomato lineFIR-A818-0433, and tomato line FDR-A815-3562V.

Tomato hybrid SVTH3452, also known as 16-A8-FIR-3452, is anindeterminate tomato variety developed for the home garden market thatdevelops a plant that produces fruit comprising the green shoulder fruittrait. Tomato hybrid SVTH3452 comprises resistance toToMV:0-2/TSWV/Fol:0/For/Sbl/Sl/Ss/Va:0/Vd:0/Ma/Mi/Mj, and also comprisesthe PEN1 and LIN5 alleles described in U.S. Pat. Pub. No. 2016/0100538.

Tomato line FIR-A818-0433 is an indeterminate tomato variety thatdevelops a plant that produces fruit comprising the green shoulder fruittrait. Tomato line FIR-A818-0433 comprises resistance toTSWV/Sbl/Sl/Ss/Va:0/Vd:0, and also comprises the PEN1 and LIN5 allelesdescribed in U.S. Pat. Pub. No. 2016/0100538.

Tomato line FDR-A815-3562V is a determinate tomato variety that developsa plant that produces uniform green fruit. Tomato line FDR-A815-3562Vcomprises resistance to ToMV: 0-2/Fol:0/For/Sbl/Sl/Ss/Va:0/Vd:0/Ma/Mi/Mj.

A. Origin and Breeding History of Tomato Hybrid SVTH3452

The parents of tomato hybrid SVTH3452 are tomato line FIR-A818-0433 andtomato line FDR-A815-3562V. The parent lines are uniform and stable, asis a hybrid produced therefrom. A small percentage of variants can occurwithin commercially acceptable limits for almost any characteristicduring the course of repeated multiplication. However no variants areexpected.

B. Physiological and Morphological Characteristics of Tomato HybridSVTH3452, Tomato Line FIR-A818-0433, and Tomato Line FDR-A815-3562V

In accordance with one aspect of the present invention, there areprovided plants having the physiological and morphologicalcharacteristics of tomato hybrid SVTH3452 and the parent lines thereof.Descriptions of the physiological and morphological characteristics ofsuch plants are presented in the tables that follow.

TABLE 1 Physiological and Morphological Characteristics of Tomato HybridSVTH3452 CHARACTERISTIC SVTH3452 PINNACLE Seedling anthocyanin inhypocotyl of 2-15 cm present present seedling habit of 3-4 week oldseedling normal normal Mature Plant height (cm) 156.27 146.40 heightlong medium growth type indeterminate indeterminate form normal lax,open canopy size (compared to others of medium large similar type) habitsemi-erect semi-erect Stem anthocyanin coloration of upper third absentor very weak weak length of internode long medium branching profuseintermediate branching at cotyledon or first leafy node present absentnumber of nodes between first 7.29 6.57 inflorescence number of nodesbetween early (first to 2.4 3.73 second, second to third) inflorescencesnumber of nodes between later developing 1.00 1.93 inflorescencespubescence on younger stems moderately hairy moderately hairy Leaf type(mature leaf beneath the third tomato tomato inflorescence) type ofblade bipinnate bipinnate margins of major leaflets (mature leafshallowly toothed or deeply toothed or cut, beneath the thirdinflorescence) scalloped sps. marginal rolling or wiltiness (mature leafmoderate absent beneath the third inflorescence) onset of leafletrolling (mature leaf mid season mid season beneath the thirdinflorescence) surface of major leaflets (mature leaf rugose rugosebeneath the third inflorescence) pubescence (mature leaf beneath thethird normal normal inflorescence) attitude horizontal horizontal lengthlong medium width broad medium size of leaflets large medium glossinessweak weak intensity of green color dark dark blistering strong mediumattitude of petiole of leaflet in relation to horizontal horizontal mainaxis Inflorescence type mainly uniparous mainly uniparous type (thirdinflorescence) simple simple average number of flowers in 5.33 5.73inflorescence (third inflorescence) leafy or ″running″ inflorescence(third frequent absent inflorescence) Flower color yellow yellow calyxmacrocalyx normal calyx-lobes approx. equaling approx. equaling corollacorolla corolla color yellow yellow style pubescence sparse sparseanthers separating into 2 or all fused into tube more groups at anthesisfasciation (first flower of second or third absent absent inflorescence)abscission layer present present length medium short Fruit surfacesmooth smooth base color (mature-green stage) light green apple ormedium green pattern (mature-green stage) green shouldered uniform greenshoulder color if different from base dark green n/a green shoulderpresent absent intensity of green color excluding light medium shoulder(before maturity) green stripes (before maturity) absent absent sizemedium medium ratio length/diameter medium small shape in longitudinalsection oblate oblong shape of blossom end flat flat shape of stem endindented indented shape of pistil scar irregular dot shape oftransverse/cross section (third round angular fruit of second or thirdcluster) ribbing at peduncle end weak weak depression at peduncle endmedium weak size of stem/peduncle scar large medium size of blossom scarlarge very small point of detachment of fruit at harvest at calyxattachment at pedicel joint (third fruit of second or third cluster)length of dedicel (third fruit of second or 15.11 12.37 thirdcluster)(mm) length (mature) (stem axis) (mm) 54.53 52.43 diameter atwidest point (mm) 73.71 63.71 weight (mature) (g) 185.2 131.6 corecoreless present diameter of core in cross section in n/a mediumrelation to total diameter number of locules 8.13 3.2 number of loculesmore than 6 two and three color (full ripe) red red flesh color(full-ripe) red/crimson red/crimson flesh color with lighter and uniformdarker areas in walls locular gel color (table-ripe) red red glossinessof skin weak medium time of flowering medium medium time of maturitylate medium firmness soft soft shelf life short short ripening(blossom-to-stem axis) uniform blossom-to-stem end ripening(peripheral-to-central-radial axis) uniformity inside out epidermiscolor yellow yellow epidermis normal easy-peel epidermis texture averagetender pericarp thickness (mm) 5.92 7.80 thickness of pericarp mediummedium sensitivity to silvering insensitive sensitive Phenology seedingto 50% flowering (1 open on 50% 61 57 of plants) (days) seeding to onceover harvest (days) 125 116 fruiting season medium medium relativematurity in areas tested late medium early Adaptation culture fieldfield principle use(s) fresh market, home fresh market, home gardengarden machine harvest not adapted not adapted regions to whichadaptation has been California: California: demonstrated Sacramento andSacramento and Upper San Joaquin Upper San Joaquin Valley ValleyChemistry and Composition pH 4.15 4.25 Titratable acidity, as % citricacid 0.711 0.45 Total solids (dry matter, seeds and skin 7.98 6.53removed) (percentage total content) Soluble solids as 'Brix 7.50 5.93These are typical values. Values may vary due to environment. Valuesthat are substantially equivalent are within the scope of the invention.

TABLE 2 Physiological and Morphological Characteristics of Tomato LineFIR-A818-0433 and Tomato Line FDR-A815-3562V CHARACTERISTICFIR-A818-0433 FDR-A815-3562V PINNACLE Seedling anthocyanin in hypocotylof 2-15 cm present present present seedling habit of 3-4 week oldseedling normal normal normal Mature Plant height (cm) 93.67 59.47100.13 growth type indeterminate determinate indeterminate height (onlyindeterminate varieties) medium n/a medium number of inflorescences onmain stem n/a medium n/a (only determinate varieties) form normal normallax, open canopy size (compared to others of medium medium large similartype) habit semi-erect semi-erect semi-erect Stem anthocyanin colorationof upper third weak absent or very weak weak length of internode longn/a medium branching intermediate intermediate intermediate branching atcotyledon or first leafy node absent absent absent number of nodesbetween first 8.43 7.50 7.50 inflorescence number of nodes between early(first to 2.20 3.20 1.20 second, second to third) inflorescences numberof nodes between later developing 4.93 0.53 2.0 inflorescencespubescence on younger stems moderately hairy moderately hairy moderatelyhairy Leaf type (mature leaf beneath the third tomato tomato tomatoinflorescence) type of blade bipinnate bipinnate bipinnate margins ofmajor leaflets (mature leaf shallowly toothed or shallowly toothed ordeeply toothed or cut, beneath the third inflorescence) scallopedscalloped sps. marginal rolling or wiltiness (mature leaf moderateslight absent beneath the third inflorescence) onset of leaflet rolling(mature leaf mid season late season mid season beneath the thirdinflorescence) surface of major leaflets (mature leaf rugose smoothrugose beneath the third inflorescence) pubescence (mature leaf beneaththe third normal normal normal inflorescence) attitude semi-erectsemi-erect semi-erect length long medium medium width medium mediummedium size of leaflets large large medium intensity of green colormedium dark dark glossiness weak weak weak blistering medium weak mediumInflorescence attitude of petiole of leaflet in relation to horizontalhorizontal horizontal main axis type mainly uniparous mainly uniparousmainly uniparous type (third inflorescence) simple simple simple averagenumber of flowers in 4.87 5.40 4.40 inflorescence (third inflorescence)leafy or ″running″ inflorescence (third occasional absent absentinflorescence) Flower color yellow yellow yellow calyx normal normalnormal calyx-lobes approx. equaling approx. equaling approx. equalingcorolla corolla corolla corolla color yellow yellow yellow stylepubescence sparse absent or very scarce absent or very scarce anthersall fused into tube all fused into tube all fused into tube fasciation(first flower of second or third absent absent absent inflorescence)abscission layer present present present length medium medium shortFruit surface smooth smooth smooth base color (mature-green stage) lightgreen apple or medium apple or medium green green pattern (mature-greenstage) green-shouldered uniform green uniform green shoulder color ifdifferent from base dark green n/a n/a green shoulder (before maturity)present absent absent extent of green shoulder (before maturity) mediumn/a n/a intensity of green color (before maturity) dark n/a n/aintensity of green color excluding light medium medium shoulder (beforematurity) green stripes (before maturity) absent absent absent sizemedium large medium ratio length/diameter medium medium small shape inlongitudinal section oblong oblong oblong shape of blossom end flatnippled flat shape of stem end indented indented indented shape ofpistil scar irregular dot dot shape of transverse/cross section (thirdround angular angular fruit of second or third cluster) ribbing atpeduncle end absent or very weak absent or very weak weak depression atpeduncle end weak absent or very weak weak size of stem/peduncle scarmedium medium medium size of blossom scar medium very small small pointof detachment of fruit at harvest at pedicel joint at pedicel joint atpedicel joint (third fruit of second or third cluster) length of dedicel(third fruit of second or 18.06 17.15 11.57 third cluster)(mm) length(mature) (stem axis) (mm) 54.92 56.36 47.1 diameter at widest point (mm)61.05 64.33 59.69 weight (mature) (g) 124.47 135.73 112.33 core presentpresent present diameter of core in cross section in medium large largerelation to total diameter number of locules 5.47 7.80 3.80 number oflocules four, five or six more than six two and three color (full ripe)red red red flesh color (full-ripe) red/crimson red/crimson red/crimsonflesh color uniform with lighter and darker uniform areas in wallslocular gel color (table-ripe) red red red time of flowering late latemedium time of maturity late medium medium glossiness of skin weakmedium medium firmness medium medium soft shelf life medium medium shortripening (blossom-to-stem axis) blossom-to-stem end uniformblossom-to-stem end ripening (peripheral-to-central-radial axis) insideout inside out inside out epidermis color yellow yellow yellow epidermiseasy-peel normal easy-peel epidermis texture tender average tenderpericarp thickness 6.20 4.44 6.51 thickness of pericarp medium thinmedium sensitivity to silvering insensitive insensitive sensitivePhenology seeding to 50% flowering 68 67 61 (1 open on 50% of plants)(days) seeding to once over harvest (days) 136 122 124 fruiting seasonshort, concentrated medium medium relative maturity in areas tested latemedium medium Adaptation culture field field field machine harvest notadapted not adapted not adapted principle use fresh market, home freshmarket, home fresh market, home garden garden garden regions to whichadaptation has been California: California: California: demonstratedSacramento and Upper San Sacramento and Upper Sacramento and UpperJoaquin Valley San Joaquin Valley San Joaquin Valley Chemistry andComposition of Full-Ripe Fruits pH 4.18 4.22 4.46 titratable acidity, as% citric acid 0.608 0.296 0.328 total solids (dry matter, skin and seeds6.68 5.50 6.56 removed) (percentage total content) soluble solids as'Brix 7.26 5.20 6.11 These are typical values. Values may vary due toenvironment. Values that are substantially equivalent are within thescope of the invention.

C. Breeding Tomato Plants

One aspect of the current invention concerns methods for producing seedof tomato hybrid SVTH3452 involving crossing tomato line FIR-A818-0433and tomato line FDR-A815-3562V. Alternatively, in other embodiments ofthe invention, tomato hybrid SVTH3452, tomato line FIR-A818-0433, ortomato line FDR-A815-3562V may be crossed with itself or with any secondplant. Such methods can be used for propagation of tomato hybridSVTH3452, tomato line FIR-A818-0433, or tomato line FDR-A815-3562V orcan be used to produce plants that are derived from tomato hybridSVTH3452, tomato line FIR-A818-0433, or tomato line FDR-A815-3562V.Plants derived from tomato hybrid SVTH3452, tomato line FIR-A818-0433,or tomato line FDR-A815-3562V may be used, in certain embodiments, forthe development of new tomato varieties.

The development of new varieties using one or more starting varieties iswell-known in the art. In accordance with the invention, novel varietiesmay be created by crossing tomato hybrid SVTH3452 followed by multiplegenerations of breeding according to such well-known methods. Newvarieties may be created by crossing with any second plant. In selectingsuch a second plant to cross for the purpose of developing novel lines,it may be desired to choose those plants which either themselves exhibitone or more selected desirable characteristics or which exhibit thedesired characteristic(s) when in hybrid combination. Once initialcrosses have been made, inbreeding and selection take place to producenew varieties. For development of a uniform line, often five or moregenerations of selfing and selection are involved.

Uniform lines of new varieties may also be developed by way ofdouble-haploids. This technique allows the creation of true breedinglines without the need for multiple generations of selfing andselection. In this manner true breeding lines can be produced in aslittle as one generation. Haploid embryos may be produced frommicrospores, pollen, anther cultures, or ovary cultures. The haploidembryos may then be doubled autonomously, or by chemical treatments(e.g., colchicine treatment). Alternatively, haploid embryos may begrown into haploid plants and treated to induce chromosome doubling. Ineither case, fertile homozygous plants are obtained. In accordance withthe invention, any of such techniques may be used in connection with aplant of the invention and progeny thereof to achieve a homozygous line.

Backcrossing can also be used to improve an inbred plant. Backcrossingtransfers a specific desirable trait from one inbred or non-inbredsource to an inbred that lacks that trait. This can be accomplished, forexample, by first crossing a superior inbred (A) (recurrent parent) to adonor inbred (non-recurrent parent), which carries the appropriate locusor loci for the trait in question. The progeny of this cross are thenmated back to the superior recurrent parent (A) followed by selection inthe resultant progeny for the desired trait to be transferred from thenon-recurrent parent. After five or more backcross generations withselection for the desired trait, the progeny have the characteristicbeing transferred, but are like the superior parent for most or almostall other loci. The last backcross generation would be selfed to givepure breeding progeny for the trait being transferred.

The plants of the present invention are particularly well suited for thedevelopment of new lines based on the elite nature of the geneticbackground of the plants. In selecting a second plant to cross withtomato hybrid SVTH3452, tomato line FIR-A818-0433, or tomato lineFDR-A815-3562V for the purpose of developing novel tomato lines, it willtypically be preferred to choose those plants which either themselvesexhibit one or more selected desirable characteristics or which exhibitthe desired characteristic(s) when in hybrid combination. Examples ofdesirable traits may include, in specific embodiments, high seed yield,high seed germination, seedling vigor, high fruit yield, diseasetolerance or resistance, and adaptability for soil and climateconditions. Consumer-driven traits, such as a fruit shape, color,texture, and taste are other examples of traits that may be incorporatedinto new lines of tomato plants developed by this invention.

Delayed fruit ripening is a trait that is especially desirable in tomatoproduction, in that it provides a number of important benefits includingan increase in fruit shelf life, the ability to transport fruit longerdistances, the reduction of spoiling of fruit during transport orstorage, and an increase in the flexibility of harvest time. The abilityto delay harvest is especially useful for the processing industry astomato fruits may be picked in a single harvest. A number of genes havebeen identified as playing a role in fruit ripening in tomato. Mutationsin some of these genes were observed to be correlated with an extendedshelf life phenotype (Gang et al., Adv. Hort. Sci. 22: 54-62, 2008). Forexample, WO2010042865 discloses that certain mutations in the 2nd or 3rdexon in the non-ripening (NOR) gene can result in extended shelf lifephenotypes. These mutations are believed to result in an early stopcodon. It is therefore expected that any mutation resulting in an earlystop codon in an exon in the NOR gene, particularly a mutation resultingin an early stop codon in the 3rd exon, will result in a slower ripeningor an extended shelf life phenotype in tomato. A marker to select forthe unique mutation of each allele and to distinguish between thedifferent alleles of the NOR gene in a breeding program can be developedby methods known in the art.

D. Further Embodiments of the Invention

In certain aspects of the invention, plants described herein areprovided modified to include at least a first desired heritable trait.Such plants may, in one embodiment, be developed by a plant breedingtechnique called backcrossing, wherein essentially all of themorphological and physiological characteristics of a variety arerecovered in addition to a genetic locus transferred into the plant viathe backcrossing technique. The term single locus converted plant asused herein refers to those tomato plants which are developed by a plantbreeding technique called backcrossing or by genetic engineering,wherein essentially all of the morphological and physiologicalcharacteristics of a variety are recovered or conserved in addition tothe single locus introduced into the variety via the backcrossing orgenetic engineering technique, respectively. By essentially all of themorphological and physiological characteristics, it is meant that thecharacteristics of a plant are recovered or conserved that are otherwisepresent when compared in the same environment, other than an occasionalvariant trait that might arise during backcrossing, introduction of atransgene, or application of a genetic engineering technique.

Backcrossing methods can be used with the present invention to improveor introduce a characteristic into the present variety. The parentaltomato plant which contributes the locus for the desired characteristicis termed the nonrecurrent or donor parent. This terminology refers tothe fact that the nonrecurrent parent is used one time in the backcrossprotocol and therefore does not recur. The parental tomato plant towhich the locus or loci from the nonrecurrent parent are transferred isknown as the recurrent parent as it is used for several rounds in thebackcrossing protocol.

In a typical backcross protocol, the original variety of interest(recurrent parent) is crossed to a second variety (nonrecurrent parent)that carries the single locus of interest to be transferred. Theresulting progeny from this cross are then crossed again to therecurrent parent and the process is repeated until a tomato plant isobtained wherein essentially all of the morphological and physiologicalcharacteristics of the recurrent parent are recovered in the convertedplant, in addition to the single transferred locus from the nonrecurrentparent.

The selection of a suitable recurrent parent is an important step for asuccessful backcrossing procedure. The goal of a backcross protocol isto alter or substitute a single trait or characteristic in the originalvariety. To accomplish this, a single locus of the recurrent variety ismodified or substituted with the desired locus from the nonrecurrentparent, while retaining essentially all of the rest of the desiredgenetic, and therefore the desired physiological and morphologicalconstitution of the original variety. The choice of the particularnonrecurrent parent will depend on the purpose of the backcross; one ofthe major purposes is to add some commercially desirable trait to theplant. The exact backcrossing protocol will depend on the characteristicor trait being altered and the genetic distance between the recurrentand nonrecurrent parents. Although backcrossing methods are simplifiedwhen the characteristic being transferred is a dominant allele, arecessive allele, or an additive allele (between recessive anddominant), may also be transferred. In this instance it may be necessaryto introduce a test of the progeny to determine if the desiredcharacteristic has been successfully transferred.

In one embodiment, progeny tomato plants of a backcross in which a plantdescribed herein is the recurrent parent comprise (i) the desired traitfrom the non-recurrent parent and (ii) all of the physiological andmorphological characteristics of tomato the recurrent parent asdetermined at the 5% significance level when grown in the sameenvironmental conditions.

New varieties can also be developed from more than two parents. Thetechnique, known as modified backcrossing, uses different recurrentparents during the backcrossing. Modified backcrossing may be used toreplace the original recurrent parent with a variety having certain moredesirable characteristics or multiple parents may be used to obtaindifferent desirable characteristics from each.

With the development of molecular markers associated with particulartraits, it is possible to add additional traits into an established germline, such as represented here, with the end result being substantiallythe same base germplasm with the addition of a new trait or traits.Molecular breeding, as described in Moose and Mumm, 2008 (PlantPhysiol., 147: 969-977), for example, and elsewhere, provides amechanism for integrating single or multiple traits or QTL into an eliteline. This molecular breeding-facilitated movement of a trait or traitsinto an elite line may encompass incorporation of a particular genomicfragment associated with a particular trait of interest into the eliteline by the mechanism of identification of the integrated genomicfragment with the use of flanking or associated marker assays. In theembodiment represented here, one, two, three or four genomic loci, forexample, may be integrated into an elite line via this methodology. Whenthis elite line containing the additional loci is further crossed withanother parental elite line to produce hybrid offspring, it is possibleto then incorporate at least eight separate additional loci into thehybrid. These additional loci may confer, for example, such traits as adisease resistance or a fruit quality trait. In one embodiment, eachlocus may confer a separate trait. In another embodiment, loci may needto be homozygous and exist in each parent line to confer a trait in thehybrid. In yet another embodiment, multiple loci may be combined toconfer a single robust phenotype of a desired trait.

Many single locus traits have been identified that are not regularlyselected for in the development of a new inbred but that can be improvedby backcrossing techniques. Single locus traits may or may not betransgenic; examples of these traits include, but are not limited to,herbicide resistance, resistance to bacterial, fungal, or viral disease,insect resistance, modified fatty acid or carbohydrate metabolism, andaltered nutritional quality. These comprise genes generally inheritedthrough the nucleus.

Direct selection may be applied where the single locus acts as adominant trait. For this selection process, the progeny of the initialcross are assayed for viral resistance or the presence of thecorresponding gene prior to the backcrossing. Selection eliminates anyplants that do not have the desired gene and resistance trait, and onlythose plants that have the trait are used in the subsequent backcross.This process is then repeated for all additional backcross generations.

Selection of tomato plants for breeding is not necessarily dependent onthe phenotype of a plant and instead can be based on geneticinvestigations. For example, one can utilize a suitable genetic markerwhich is closely genetically linked to a trait of interest. One of thesemarkers can be used to identify the presence or absence of a trait inthe offspring of a particular cross, and can be used in selection ofprogeny for continued breeding. This technique is commonly referred toas marker assisted selection. Any other type of genetic marker or otherassay which is able to identify the relative presence or absence of atrait of interest in a plant can also be useful for breeding purposes.Procedures for marker assisted selection are well known in the art. Suchmethods will be of particular utility in the case of recessive traitsand variable phenotypes, or where conventional assays may be moreexpensive, time consuming, or otherwise disadvantageous. In addition,marker assisted selection may be used to identify plants comprisingdesirable genotypes at the seed, seedling, or plant stage, to identifyor assess the purity of a cultivar, to catalog the genetic diversity ofa germplasm collection, and to monitor specific alleles or haplotypeswithin an established cultivar.

Types of genetic markers which could be used in accordance with theinvention include, but are not necessarily limited to, Simple SequenceLength Polymorphisms (SSLPs) (Williams et al., Nucleic Acids Res., 18:6531-6535, 1990), Randomly Amplified Polymorphic DNAs (RAPDs), DNAAmplification Fingerprinting (DAF), Sequence Characterized AmplifiedRegions (SCARs), Arbitrary Primed Polymerase Chain Reaction (AP-PCR),Amplified Fragment Length Polymorphisms (AFLPs) (EP 534 858,specifically incorporated herein by reference in its entirety), andSingle Nucleotide Polymorphisms (SNPs) (Wang et al., Science,280:1077-1082, 1998).

In particular embodiments of the invention, marker assisted selection isused to increase the efficiency of a backcrossing breeding scheme forproducing a tomato line comprising a desired trait. This technique iscommonly referred to as marker assisted backcrossing (MABC). Thistechnique is well-known in the art and may involve, for example, the useof three or more levels of selection, including foreground selection toidentity the presence of a desired locus, which may complement orreplace phenotype screening protocols; recombinant selection to minimizelinkage drag; and background selection to maximize recurrent parentgenome recovery.

E. Plants Derived by Genetic Engineering

Various genetic engineering technologies have been developed and may beused by those of skill in the art to introduce traits in plants. Incertain aspects of the claimed invention, traits are introduced intotomato plants via altering or introducing a single genetic locus ortransgene into the genome of a recited variety or progenitor thereof.Methods of genetic engineering to modify, delete, or insert genes andpolynucleotides into the genomic DNA of plants are well-known in theart.

In specific embodiments of the invention, improved tomato lines can becreated through the site-specific modification of a plant genome.Methods of genetic engineering include, for example, utilizingsequence-specific nucleases such as zinc-finger nucleases (see, forexample, U.S. Pat. Appl. Pub. No. 2011-0203012); engineered or nativemeganucleases; TALE-endonucleases (see, for example, U.S. Pat. Nos.8,586,363 and 9,181,535); and RNA-guided endonucleases, such as those ofthe CRISPR/Cas systems (see, for example, U.S. Pat. Nos. 8,697,359 and8,771,945 and U.S. Pat. Appl. Pub. No. 2014-0068797). One embodiment ofthe invention thus relates to utilizing a nuclease or any associatedprotein to carry out genome modification. This nuclease could beprovided heterologously within donor template DNA for templated-genomicediting or in a separate molecule or vector. A recombinant DNA constructmay also comprise a sequence encoding one or more guide RNAs to directthe nuclease to the site within the plant genome to be modified. Furthermethods for altering or introducing a single genetic locus include, forexample, utilizing single-stranded oligonucleotides to introduce basepair modifications in a tomato plant genome (see, for example Sauer etal., Plant Physiol, 170(4):1917-1928, 2016).

Methods for site-directed alteration or introduction of a single geneticlocus are well-known in the art and include those that utilizesequence-specific nucleases, such as the aforementioned, or complexes ofproteins and guide-RNA that cut genomic DNA to produce a double-strandbreak (DSB) or nick at a genetic locus. As is well-understood in theart, during the process of repairing the DSB or nick introduced by thenuclease enzyme, a donor template, transgene, or expression cassettepolynucleotide may become integrated into the genome at the site of theDSB or nick. The presence of homology arms in the DNA to be integratedmay promote the adoption and targeting of the insertion sequence intothe plant genome during the repair process through homologousrecombination or non-homologous end joining (NHEJ).

In another embodiment of the invention, genetic transformation may beused to insert a selected transgene into a plant of the invention ormay, alternatively, be used for the preparation of transgenes which canbe introduced by backcrossing. Methods for the transformation of plantsthat are well-known to those of skill in the art and applicable to manycrop species include, but are not limited to, electroporation,microprojectile bombardment, Agrobacterium-mediated transformation, anddirect DNA uptake by protoplasts.

To effect transformation by electroporation, one may employ eitherfriable tissues, such as a suspension culture of cells or embryogeniccallus or alternatively one may transform immature embryos or otherorganized tissue directly. In this technique, one would partiallydegrade the cell walls of the chosen cells by exposing them topectin-degrading enzymes (pectolyases) or mechanically wound tissues ina controlled manner.

An efficient method for delivering transforming DNA segments to plantcells is microprojectile bombardment. In this method, particles arecoated with nucleic acids and delivered into cells by a propellingforce. Exemplary particles include those comprised of tungsten,platinum, and preferably, gold. For the bombardment, cells in suspensionare concentrated on filters or solid culture medium. Alternatively,immature embryos or other target cells may be arranged on solid culturemedium. The cells to be bombarded are positioned at an appropriatedistance below the macroprojectile stopping plate.

An illustrative embodiment of a method for delivering DNA into plantcells by acceleration is the Biolistics Particle Delivery System, whichcan be used to propel particles coated with DNA or cells through ascreen, such as a stainless steel or Nytex screen, onto a surfacecovered with target cells. The screen disperses the particles so thatthey are not delivered to the recipient cells in large aggregates.Microprojectile bombardment techniques are widely applicable, and may beused to transform virtually any plant species.

Agrobacterium-mediated transfer is another widely applicable system forintroducing gene loci into plant cells. An advantage of the technique isthat DNA can be introduced into whole plant tissues, thereby bypassingthe need for regeneration of an intact plant from a protoplast. ModernAgrobacterium transformation vectors are capable of replication in E.coli as well as Agrobacterium, allowing for convenient manipulations(Klee et al., Nat. Biotechnol., 3(7):637-642, 1985). Moreover, recenttechnological advances in vectors for Agrobacterium-mediated genetransfer have improved the arrangement of genes and restriction sites inthe vectors to facilitate the construction of vectors capable ofexpressing various polypeptide coding genes. The vectors described haveconvenient multi-linker regions flanked by a promoter and apolyadenylation site for direct expression of inserted polypeptidecoding genes. Additionally, Agrobacterium containing both armed anddisarmed Ti genes can be used for transformation.

In those plant strains where Agrobacterium-mediated transformation isefficient, it is the method of choice because of the facile and definednature of the gene locus transfer. The use of Agrobacterium-mediatedplant integrating vectors to introduce DNA into plant cells is wellknown in the art (Fraley et al., Nat. Biotechnol., 3:629-635, 1985; U.S.Pat. No. 5,563,055).

Transformation of plant protoplasts also can be achieved using methodsbased on calcium phosphate precipitation, polyethylene glycol treatment,electroporation, and combinations of these treatments (see, for example,Potrykus et al., Mol. Gen. Genet., 199:183-188, 1985; Omirulleh et al.,Plant Mol. Biol., 21(3):415-428, 1993; Fromm et al., Nature,312:791-793, 1986; Uchimiya et al., Mol. Gen. Genet., 204:204, 1986;Marcotte et al., Nature, 335:454, 1988). Transformation of plants andexpression of foreign genetic elements is exemplified in Choi et al.(Plant Cell Rep., 13:344-348, 1994), and Ellul et al. (Theor. Appl.Genet., 107:462-469, 2003).

A number of promoters have utility for plant gene expression for anygene of interest including but not limited to selectable markers,scoreable markers, genes for pest tolerance, disease resistance,nutritional enhancements and any other gene of agronomic interest.Examples of constitutive promoters useful for plant gene expressioninclude, but are not limited to, the cauliflower mosaic virus (CaMV)P-35S promoter, which confers constitutive, high-level expression inmost plant tissues (see, for example, Odel et al., Nature, 313:810,1985), including in monocots (see, for example, Dekeyser et al., PlantCell, 2:591, 1990; Terada and Shimamoto, Mol. Gen. Genet., 220:389,1990); a tandemly duplicated version of the CaMV 35S promoter, theenhanced 35S promoter (P-e35S); the nopaline synthase promoter (An etal., Plant Physiol., 88:547, 1988); the octopine synthase promoter(Fromm et al., Plant Cell, 1:977, 1989); the figwort mosaic virus(P-FMV) promoter as described in U.S. Pat. No. 5,378,619; an enhancedversion of the FMV promoter (P-eFMV) where the promoter sequence ofP-FMV is duplicated in tandem; the cauliflower mosaic virus 19Spromoter; a sugarcane bacilliform virus promoter; a commelina yellowmottle virus promoter; and other plant virus promoters known to expressin plant cells.

A variety of plant gene promoters that are regulated in response toenvironmental, hormonal, chemical, or developmental signals can also beused for expression of an operably linked gene in plant cells, includingpromoters regulated by (1) heat (Callis et al., Plant Physiol., 88:965,1988), (2) light (e.g., pea rbcS-3A promoter, Kuhlemeier et al., PlantCell, 1:471, 1989; maize rbcS promoter, Schaffner and Sheen, Plant Cell,3:997, 1991; or chlorophyll a/b-binding protein promoter, Simpson etal., EMBO J., 4:2723, 1985), (3) hormones, such as abscisic acid(Marcotte et al., Plant Cell, 1:969, 1989), (4) wounding (e.g., wunl,Siebertz et al., Plant Cell, 1:961, 1989); or (5) chemicals, such asmethyl jasmonate, salicylic acid, or Safener. It may also beadvantageous to employ organ-specific promoters (e.g., Roshal et al.,EMBO J., 6:1155, 1987; Schernthaner et al., EMBO J., 7:1249, 1988;Bustos et al., Plant Cell, 1:839, 1989).

Exemplary nucleic acids which may be introduced to plants of thisinvention include, for example, DNA sequences or genes from anotherspecies, or even genes or sequences which originate with or are presentin the same species, but are incorporated into recipient cells bygenetic engineering methods rather than classical reproduction orbreeding techniques. However, the term “exogenous” is also intended torefer to genes that are not normally present in the cell beingtransformed, or perhaps simply not present in the form, structure, etc.,as found in the transforming DNA segment or gene, or genes which arenormally present and that one desires to express in a manner thatdiffers from the natural expression pattern, e.g., to over-express.Thus, the term “exogenous” gene or DNA is intended to refer to any geneor DNA segment that is introduced into a recipient cell, regardless ofwhether a similar gene may already be present in such a cell. The typeof DNA included in the exogenous DNA can include DNA which is alreadypresent in the plant cell, DNA from another plant, DNA from a differentorganism, or a DNA generated externally, such as a DNA sequencecontaining an antisense message of a gene, or a DNA sequence encoding asynthetic or modified version of a gene.

Many hundreds if not thousands of different genes are known and couldpotentially be introduced into a tomato plant according to theinvention. Non-limiting examples of particular genes and correspondingphenotypes one may choose to introduce into a tomato plant include oneor more genes for insect tolerance, such as a Bacillus thuringiensis(B.t.) gene, pest tolerance such as genes for fungal disease control,herbicide tolerance such as genes conferring glyphosate tolerance, andgenes for quality improvements such as yield, nutritional enhancements,environmental or stress tolerances, or any desirable changes in plantphysiology, growth, development, morphology or plant product(s). Forexample, structural genes would include any gene that confers insecttolerance including but not limited to a Bacillus insect control proteingene as described in WO 99/31248, herein incorporated by reference inits entirety, U.S. Pat. No. 5,689,052, herein incorporated by referencein its entirety, U.S. Pat. Nos. 5,500,365 and 5,880,275, hereinincorporated by reference in their entirety. In another embodiment, thestructural gene can confer tolerance to the herbicide glyphosate asconferred by genes including, but not limited to Agrobacterium strainCP4 glyphosate resistant EPSPS gene (aroA:CP4) as described in U.S. Pat.No. 5,633,435, herein incorporated by reference in its entirety, orglyphosate oxidoreductase gene (GOX) as described in U.S. Pat. No.5,463,175, herein incorporated by reference in its entirety.

Alternatively, the DNA coding sequences can affect these phenotypes byencoding a non-translatable RNA molecule that causes the targetedinhibition of expression of an endogenous gene, for example viaantisense- or cosuppression-mediated mechanisms (see, for example, Birdet al., Biotech. Gen. Engin. Rev., 9:207, 1991). The RNA could also be acatalytic RNA molecule (i.e., a ribozyme) engineered to cleave a desiredendogenous mRNA product (see, for example, Gibson and Shillito, Mol.Biotech., 7:125, 1997). Thus, any gene which produces a protein or mRNAwhich expresses a phenotype or morphology change of interest is usefulfor the practice of the present invention.

F. Definitions

In the description and tables herein, a number of terms are used. Inorder to provide a clear and consistent understanding of thespecification and claims, the following definitions are provided:

Allele: Any of one or more alternative forms of a genetic locus, all ofwhich alleles relate to one trait or characteristic. In a diploid cellor organism, the two alleles of a given gene occupy corresponding locion a pair of homologous chromosomes.

Backcrossing: A process in which a breeder repeatedly crosses hybridprogeny, for example a first generation hybrid (F₁), back to one of theparents of the hybrid progeny. Backcrossing can be used to introduce oneor more single locus conversions or transgenes from one geneticbackground into another.

Crossing: The mating of two parent plants.

Cross-Pollination: Fertilization by the union of two gametes fromdifferent plants.

Diploid: A cell or organism having two sets of chromosomes.

Emasculate: The removal of plant male sex organs or the inactivation ofthe organs with a cytoplasmic or nuclear genetic factor or a chemicalagent conferring male sterility.

Enzymes: Molecules which can act as catalysts in biological reactions.

F₁ Hybrid: The first generation progeny of the cross of two nonisogenicplants.

Genotype: The genetic constitution of a cell or organism.

Haploid: A cell or organism having one set of the two sets ofchromosomes in a diploid.

Linkage: A phenomenon wherein alleles on the same chromosome tend tosegregate together more often than expected by chance if theirtransmission was independent.

Marker: A readily detectable phenotype, preferably inherited incodominant fashion (both alleles at a locus in a diploid heterozygoteare readily detectable), with no environmental variance component, i.e.,heritability of 1.

Phenotype: The detectable characteristics of a cell or organism, whichcharacteristics are the manifestation of gene expression.

Quantitative Trait Loci (QTL): Quantitative trait loci (QTL) refer togenetic loci that control to some degree numerically representabletraits that are usually continuously distributed.

Resistance: As used herein, the terms “resistance” and “tolerance” areused interchangeably to describe plants that show no symptoms to aspecified biotic pest, pathogen, abiotic influence, or environmentalcondition. These terms are also used to describe plants showing somesymptoms but that are still able to produce marketable product with anacceptable yield. Some plants that are referred to as resistant ortolerant are only so in the sense that they may still produce a crop,even though the plants are stunted and the yield is reduced.

Regeneration: The development of a plant from tissue culture.

Royal Horticultural Society (RHS) Color Chart Value: The RHS Color Chartis a standardized reference which allows accurate identification of anycolor. A color's designation on the chart describes its hue, brightness,and saturation. A color is precisely named by the RHS Color Chart byidentifying the group name, sheet number, and letter, e.g.,Yellow-Orange Group 19A or Red Group 41B.

Self-Pollination: The transfer of pollen from the anther to the stigmaof the same plant.

Single Locus Converted (Conversion) Plant: Plants which are developed bya plant breeding technique called backcrossing or genetic engineering ofa locus, wherein essentially all of the morphological and physiologicalcharacteristics of a tomato variety are recovered in addition to thecharacteristics of the single locus.

Substantially Equivalent: A characteristic that, when compared, does notshow a statistically significant difference (e.g., p=0.05) from themean.

Tissue Culture: A composition comprising isolated cells of the same or adifferent type or a collection of such cells organized into parts of aplant.

Transgene: A genetic locus comprising a sequence which has beenintroduced into the genome of a tomato plant by transformation orsite-specific modification.

G. Deposit Information

A deposit of tomato line FIR-A818-0433 and tomato line FDR-A815-3562V,disclosed above and recited in the claims, has been made with theProvasoli-Guillard National Center for Marine Algae and Microbiota(NCMA), 60 Bigelow Drive, East Boothbay, Me., 04544 USA. The date ofdeposit for those deposited seeds of tomato line FIR-A818-0433 andtomato line FDR-A815-3562V are Jun. 9, 2020 and Jun. 17, 2020,respectively. The accession numbers for those deposited seeds of tomatoline FIR-A818-0433 and tomato line FDR-A815-3562V are NCMA Accession No.202006002 and NCMA Accession No. 202006019, respectively. Upon issuanceof a patent, all restrictions upon the deposits will be removed, and thedeposits are intended to meet all of the requirements of 37 C.F.R. §§1.801-1.809. The deposits have been accepted under the Budapest Treatyand will be maintained in the depository for a period of 30 years, 5years after the last request, or the effective life of the patent,whichever is longer, and will be replaced if necessary during thatperiod.

Although the foregoing invention has been described in some detail byway of illustration and example for purposes of clarity andunderstanding, it will be obvious that certain changes and modificationsmay be practiced within the scope of the invention, as limited only bythe scope of the appended claims.

All references cited herein are hereby expressly incorporated herein byreference.

What is claimed:
 1. A tomato plant comprising at least a first set ofthe chromosomes of tomato line FIR-A818-0433, a sample of seed of saidline having been deposited under NCMA Accession No.
 202006002. 2. Atomato seed that produces the plant of claim
 1. 3. The plant of claim 1,wherein the plant is an inbred plant of said tomato line FIR-A818-0433.4. The seed of claim 2, wherein the seed is an inbred seed of saidtomato line FIR-A818-0433.
 5. A plant part of the plant of claim 1,wherein the plant part comprises a cell of said plant.
 6. A tomato planthaving all of the physiological and morphological characteristics of theplant of claim
 3. 7. A tissue culture of regenerable cells of the plantof claim
 1. 8. A method of vegetatively propagating the plant of claim1, the method comprising the steps of: (a) collecting tissue capable ofbeing propagated from the plant of claim 1; and (b) propagating a tomatoplant from said tissue.
 9. A method of introducing a trait into a tomatoline, the method comprising: (a) utilizing as a recurrent parent theplant of claim 3 by crossing said plant with a donor plant thatcomprises a trait to produce F₁ progeny; (b) selecting an F₁ progenythat comprises the trait; (c) backcrossing the selected F₁ progeny witha plant of the same line used as the recurrent parent in step (a) toproduce backcross progeny; (d) selecting a backcross progeny comprisingthe trait and otherwise comprising the morphological and physiologicalcharacteristics of the recurrent parent line used in step (a); and (e)repeating steps (c) and (d) three or more times to produce a selectedfourth or higher backcross progeny.
 10. A tomato plant produced by themethod of claim 9, wherein said plant comprises the trait and otherwisecomprises all of the physiological and morphological characteristics oftomato line FIR-A818-0433.
 11. A method of producing a tomato plantcomprising an added trait, the method comprising introducing a transgeneconferring the trait into the plant of claim
 1. 12. A tomato plantproduced by the method of claim 11, wherein said plant comprises thetrait and otherwise comprises all of the physiological and morphologicalcharacteristics of tomato line FIR-A818-0433.
 13. A tomato plantcomprising at least a first set of the chromosomes of tomato lineFIR-A818-0433, a sample of seed of said line having been deposited underNCMA Accession No. 202006002, further comprising a transgene.
 14. Theplant of claim 13, wherein the transgene confers a trait selected fromthe group consisting of male sterility, herbicide tolerance, insectresistance, pest resistance, disease resistance, modified fatty acidmetabolism, environmental stress tolerance, modified carbohydratemetabolism, and modified protein metabolism.
 15. A tomato plantcomprising at least a first set of the chromosomes of tomato lineFIR-A818-0433, a sample of seed of said line having been deposited underNCMA Accession No. 202006002, further comprising a single locusconversion.
 16. The plant of claim 15, wherein the single locusconversion confers a trait selected from the group consisting of malesterility, herbicide tolerance, insect resistance, pest resistance,disease resistance, modified fatty acid metabolism, environmental stresstolerance, modified carbohydrate metabolism, and modified proteinmetabolism.
 17. A method for producing a seed of a tomato plant derivedfrom tomato line FIR-A818-0433, the method comprising the steps of: (a)crossing the plant of claim 1 with itself or a different tomato plant;and (b) allowing a seed of a tomato line FIR-A818-0433-derived tomatoplant to form.
 18. A method of producing a seed of a tomato lineFIR-A818-0433-derived tomato plant, the method comprising the steps of:(a) producing a tomato line FIR-A818-0433-derived tomato plant from aseed produced by crossing the plant of claim 1 with itself or adifferent tomato plant; and (b) crossing the tomato lineFIR-A818-0433-derived tomato plant with itself or a different tomatoplant to obtain a seed of a further tomato line FIR-A818-0433-derivedtomato plant.
 19. The method of claim 18, the method further comprisingrepeating said producing and crossing steps of (a) and (b) using theseed from said step (b) for at least one generation to produce a seed ofan additional tomato line FIR-A818-0433-derived tomato plant.
 20. Amethod of producing a tomato fruit, the method comprising: (a) obtainingthe plant of claim 1, wherein the plant has been cultivated to maturity;and (b) collecting a tomato fruit from the plant.